A novel strategy for triacylglycerides and polyhydroxyalkanoates production using waste lipids

Lipids are one of the main components organic matter present in effluents food-processing industry. These waste streams can be biotransformed into valuable triacylglycerides (TAGs) and polyhydroxyalkanoates (PHAs), precursors biofuels biomaterials alternative to petroleum-based products. compounds yielded by mixed microbial cultures, considering that both TAG PHA accumulators may coexist within community, it seems crucial define those operational strategies might control selection dominant metabolic pathways (TAG or accumulation). In this work, residual fish-canning oil was used as a carbon source two-stage process (culture intracellular accumulation) which substrate simultaneously hydrolyzed these two stages without need for previous fermentation unit. It pretended maximize preferential storage accumulation reactor imposition certain selective pressures enrichment one. Uncoupling C N feedings limiting nitrogen availability medium, allowed production (82.3 wt% PHAs, 0.80 Cmmol /Cmmol S ). Besides, when low pH famine phase considered additional pressure, possible shift ratio TAG:PHA from 4:96 obtaining 43.0 TAGs (0.67 Therefore, novel simplified demonstrated versatility efficiency PHAs unique feedstock using an open culture proving product harnessed if choosing right conditions stage. • Preferent lipids No pretreatment needed before stages. Dominant depended on imposed pressures. Carbon (limited) uncoupling promoted storage. Low famine, advantage triacylglyceride-accumulators